Study of the potential toxicity of adrenaline to neurons, using the SH-SY5Y human cellular model

Abstract Prolonged overexposure to catecholamines causes toxicity, usually credited to continuous adrenoceptor stimulation, autoxidation, and the formation of reactive pro-oxidant species. Non-differentiated SH-SY5Y cells were used to study the possible contribution of oxidative stress in adrenaline (ADR)-induced neurotoxicity, as a model to predict the toxicity of this catecholamine to peripheral nerves. Cells were exposed to several concentrations of ADR (0.1, 0.25, 0.5 and 1mM) and two cytotoxicity assays [lactate dehydrogenase (LDH) release and 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl tetrazolium bromide (MTT) reduction] were performed at several time-points (24, 48, and 96h). The cytotoxicity of ADR was concentration- and time-dependent in both assays, since the lowest concentration tested (0.1mM) also caused significant cytotoxicity at 96h. N-acetyl-cysteine (1mM), a precursor of glutathione synthesis, prevented ADR-induced toxicity elicited by 0.5mM and 0.25mM ADR following a 96-h exposure, while the antioxidant Tiron (100µM) was non-protective. In conclusion, ADR led to mitochondrial distress and ultimately cell death in non-differentiated SH-SY5Y cells, possibly because of ADR oxidation products. The involvement of such processes in the catecholamine-induced peripheral neuropathy requires further analysis.

Heterobostrychus aequalis (Waterhouse, 1884) (Coleoptera: Bostrichidae): Its interception at the Harbor of Rio de Janeiro and relevance as a quarentine pest (A1)

Heterobostrychus aequalis (Waterhouse, 1884) (Coleoptera: Bostrichidae) is considered a severe pest for wood and wood products in regions where it is established. In Brazil, so far, there are no records of its establishment. Therefore, this work reports the interception of this Bostrichidae in the Harbor of Rio de Janeiro, on pallet wood from India. It also defends the maintenance of this insect as an absent quarantine pest (A1), by the Ministry of Agriculture, Livestock and Supply. It also conducts a discussion that addresses the efficiency of wood treatments, usually used to prevent the spread of quarantine pests in environments where there is international transit of wood, demonstrating that they may not be efficient in this regard, especially for insect species that have the capacity to lay eggs on dry wood. In this context, it also suggests population monitoring, combined with inspections, as an aid measure for the early detection of this pest in an environment where there is international transit of wood.

Bromoderma in an infant

Abstract Bromoderma is a cutaneous eruption caused by the absorption of bromide. Clinical manifestations include acneiform and vegetative lesions. We report the case of an infant with bromoderma caused by the use of syrup for abdominal colic containing calcium bromide. The lesions regressed after discontinuation of the drug.

Preparation and physical properties of [PVA][0.7] [NaBr][0.3] [H[3]PO[4]][xM] solid acid membrane for phosphoric acid - Fuel cells

A solid acid membranes based on poly [vinyl alcohol] [PVA], sodium bromide [NaBr] and phosphoric acid [H[3]PO[4]] were prepared by a solution casting method. The morphological, IR, electrical and optical properties of the [PVA][0.7][NaBr][0.3][H[3]PO[4]][xM] solid acid membranes where x =0.00, 0.85, 1.7, 3.4, 5.1 M were investigated. The variation of film morphology was examined by scanning electron microscopy [SEM] studies. FTIR spectroscopy has been used to characterize the structure of polymer and confirms the complexation of phosphoric acid with host polymeric matrix. The temperature dependent nature of ionic conductivity and the impedance of the polymer electrolytes were determined along with the associated activation energy. The ionic conductivity at room temperature was found to be strongly depends on the H[3]PO[4] concentration which it has been achieved to be of the order 4.3 x 10[-3] S/cm at ambient temperature. Optical measurements showed a decrease in optical band gap and an increase in band tail width with the increase of phosphoric acid. The data shows that the [PVA][0.7][NaBr][0.3][H[3]PO[4]][xM] solid acid membrane is promising for intermediate temperature phosphoric acid fuel cell applications

Cloning and Sequence Analysis of the Cellobiohydrolase I Genes from Some Basidiomycetes

Genes encoding the cellobiohydrolase enzyme (CBHI), designated as cbhI, were isolated from the basidiomycetes Auricularia fuscosuccinea, Pleurotus giganteus, P. eryngii, P. ostreatus, and P. sajor-caju. Initially, the fungal genomic DNA was extracted using a modified cetyltrimethyl ammonium bromide (CTAB) protocol and used as a DNA template. The cbhI genes were then amplified and cloned using the pGEM-T Easy Vector Systems. The sizes of these PCR amplicons were between 700~800 bp. The DNA sequences obtained were similar showing high identity to the cbhI gene family. These cbhI genes were partial consisting of three coding regions and two introns. The deduced amino acid sequences exhibited significant similarity to those of fungal CBHI enzymes belonging to glycosyl hydrolase family 7.

Arranjos supramoleculares de oligodeoxinucleotídeos e fragmentos de bicamada catiônica: preparação, caracterização e atividade imunoadjuvante / Supramolecular assemblies of oligodeoxynucleotides and cationic bilayer fragments: preparation, characterization and immunoadjuvant activity

A interação entre fragmentos de bicamada (BF) de brometo de dioctadecildimetilamônio (DODAB) e um mononucleotídeo-modelo (deoxiadenosina monofosfato, dAMP) ou um oligodeoxinucleotídeo-modelo (5'- AAAAAAAAAA-3', poli(dA)) ou um oligodeoxinucleotídeo terapêutico (5'- TTGACGTTCG -3', CpG) foi investigada por turbidimetria, espalhamento de luz dinâmico, espectroscopia de dicroísmo circular e de fluorescência e calorimetria diferencial de varredura (DSC). Respostas imunológicas foram caracterizadas com ensaio de hipersensibilidade tardia por inchamento de coxim patelar de camundongo, dosagem de anticorpos IgG1 e IgG2a e de citocinas secretadas por células de linfonodo em cultura. Poli(dA), em contraste com dAMP, induziu fusão máxima de DODAB BF a partir da neutralização de cargas, quando houve obtenção de um tamanho máximo e um potencial-zeta igual a zero para os arranjos. Para [poli(dA)] maiores do que aquela correspondente à neutralização de cargas, houve recuperação da estabilidade coloidal com reversão do potencial-zeta e com obtenção de tamanhos que foram aproximadamente o dobro daqueles determinados inicialmente para DODAB BF. A proporção molar de neutralização poli(dA): DODAB foi 1:10 para DODAB BF e 1:20 para vesículas grandes (LV) de DODAB, de acordo com as estruturas de bicamada aberta e fechada dessas duas dispersões de bicamada de DODAB. A fusão de DODAB BF induzida por poli(dA) foi extensiva aumentando o grau de empacotamento das bicamadas formadas conforme inferido a partir dos termogramas de DSC. Em condições de equivalencia de cargas, nucleotídeo não causou fusão de DODAB BF, mostrando a importância do caráter de polieletrólito do poli(dA) para induzir fusão. O sal divalente Na2HPO4 causou fusão e aumentou o empacotamento da bicamada graças à blindagem eficiente de cargas. Reestabilização coloidal como aquela induzida por poli(dA) não ocorreu em presença de Na2HPO4, NaCl ou nucleotídeo. Para complexos DODAB BF/CpG em presença de ovalbumina (OVA)...

The interaction between bilayer fragments (BF) of dioctadecyldimethylammonium bromide (DODAB) and a model nucleotide (deoxyadenosine monophosphate, dAMP) or a model oligodeoxynucleotide (5'- AAAAAAAAAA-3', poly(dA)) or a therapeutic oligodeoxynucleotide (5'- TTGACGTTCG -3', CpG) was investigated by means of turbidimetry, dynamic light scattering, circular dichroism and fluorescence spectroscopies and differential scanning calorimetry. Immune responses were characterized using footpad swelling delayed type hipersensitivity assay and antibody and cytokine measurements. In contrast to dAMP, poly(dA) induced maximal DODAB BF fusion from charge neutralization, where assemblies presented maximal size and zero zeta-potential. Above charge neutralization colloid stability was recovered with negative zeta-potentials and sizes that were about the double of those initially determined for DODAB BF. The poly(dA):DODAB molar ratio for neutralization was 1:10 for DODAB BF and 1:20 for DODAB LV, in agreement with the open and closed bilayer structures of these two DODAB bilayer dispersions. The poly(dA)-induced DODAB BF fusion was extensive and increased the packing of the formed bilayers, as inferred from DSC thermograms. In conditions of charge equivalence, nucleotide did not cause DODAB BF fusion, highlighting the importance of poly(dA)'s polyelectrolyte character to induce fusion. Divalent Na2HPO4 salt caused fusion and increased bilayer packing due to efficient BF charge shielding. Colloid restabilization as induced by poly(dA) was not observed in presence of Na2HPO4, NaCl and nucleotide. For DODAB BF/CpG complexes in presence of the ovalbumin (OVA) model antigen, the charge neutralization of DODAB BF/OVA by CpG reduced colloid stability, while charge overcompensation led to restabilization due to electrostatic repulsion, as observed for DODAB BF/poly(dA) interaction. Differences in size and neutralization proportions by CpG indicate that BF are able to load more OVA…

Cloreto e status hidroeletrolítico e ácido-básico: importância clínica da determinação laboratorial / Chloride and hydroelectrolytic and acid base status: clinical importance of laboratorial determination

Apesar de o cloreto ser um exame amplamente disponível e solicitado em instituições de saúde, a maior pane dos médicos crê ser difícil interpretar seus resultados, estabelecer correlações com outros parâmetros laboratoriais associados e tomar decisões terapêuticas baseadas no mesmo. Devido à complexidade de sua homeostase e à intrincada correlação com o status hidroeletrolítico e ácido-básico, poucos médicos se sentem efetivamente aptos a aproveitar a valiosa informação clínica que ele pode revelar. Isto é agravado pela rarefeita bibliografia objetiva sobre o tema. Os autores desta revisão não foram capazes de encontrar nenhum capítulo especificamente dedicado ao cloreto nos principais livros-texto de Fisiologia, Clínica Médica e Nefrologia, disponíveis, à exceção de três revisões bibliográficas no Medline. Não obstante, tentamos organizar a informação tão claramente quanto possível, com o objetivo de tornar o cloreto uma ferramenta útil aos nossos colegas profissionais de saúde.

Even though chloride is a widely available and requested test in health institutions, most part of physicians find it difficult to interpret its results, establish correlations with other laboratory linked parameters and take therapeutic decisions based on it. Due to the complexity of its homeostatic balance and intrincated correlation to hydroelectrolytic and acid base status, few doctors feel actually able to fully profit from the valuable clinical information it can unfold. This is aggravated by the scarce objective bibliography on the issue. The authors of this review were not able to find any chapters specifically dedicated to chloride on major Physiology, Internal Medicine and Nephrology textbooks, but only three reviews on Medline. Nevertheless, we managed to organize the information as clearly as possible with the aim of making chloride test an useful tool to our fellow health professionals.

Sensitive and rapid titrimetric and spectrophotometric methods for the determination of stavudine in pharmaceuticals using bromate-bromide and three dyes

Four sensitive and rapid methods for the determination of stavudine (STV) in bulk drug and in dosage forms were developed and optimized. In titrimetry, aqueous solution of STV was treated with a known excess of bromate-bromide in HCl medium followed by estimation of unreacted bromine by iodometric back titration. Spectrophotometric methods involve the addition of a measured excess of bromate-bromide in HCl medium and subsequent estimation of the residual bromine by reacting with a fixed amount of methyl orange, indigocarmine or thymol blue followed by measurement of absorbance at 520 nm (method A), 610 nm (method B) or 550 nm (method C). In all the methods, the amount of bromate reacted corresponds to the amount of STV. Calculations in titrimetry were based on a 1:0.666 (STV:KBrO3) stoichiometry and the method was found to be applicable over 3.5-10 mg range. A linear increase in absorbance with concentration of STV was observed in the spectrophotometric methods, and the Beer's law was obeyed over the concentration ranges 0.125-1.75, 1-10 and 1-9.0 µg mL-1 STV for method A, method B and method C, respectively. The methods when applied to the determination of STV in tablets and capsules were found to give satisfactory results.

Este trabalho descreve quatro métodos rápidos e sensíveispara a determinação de estavudina (STV) na matéria-prima ou em produtos formulados. Soluções aquosas de STV podem ser tituladas tratando-as com excesso de bromato-brometo em meio ácido clorídrico, seguido da determinação iodimétrica de bromo em excesso. Métodos espectrofotométricos tambémenvolvem a adição de excesso de bromato-brometo à amostra, seguida da determinação de bromo residual por adição de uma quantidade fixa de alaranjado de metila, índigo-carmim ou azul de timol, e de medidas de absorbância nos comprimentos de onda apropriados: 520, 610 ou 550 nm. Em todos os métodos, a quantidade de bromato consumida corresponde à quantidade de STV e os resultados da sua aplicação à determinação de STV em comprimidos e cápsulas são satisfatórios.

Some chemical and physical characteristics of Alexandria coastal water, Egypt

Seasonal collection of water samples were carried out at Alexandria coast from 18 selected stations during 2004 - 2005. The study area was divided into three sections A, B, and C. The study areas A and C are clearly affected by brackish polluted water discharge from El-Ammia, Rakta, Bughas, Umum, and Noubaria drains which were studied too. The hydrographic parameters [pH, DO, salinity, and OOM] were studied. This work aimed at evaluating some major ions level [average of 434.6 mg/l, 1.39 g/l, 3.11g/l, and 67.32 mg/l for Ca, Mg, SO[4], and Br in surface water and 449.12 mg/l, 1.48g/l, 3.42 g/l, and 75.68 mg/l in bottom water, respectively] in Alexandria coastal water and their chlorinity ratios relevant to the corresponding normal oceanic ones. The influence of dilution effect resulted from the discharge of different types and amount of land-based effluents was discussed. By all, the average chlorinity ratios of cations deviations from the respective oceanic values can detect as a resulting from the discharging of land -based effluents; [average of 0.074, 0.023, 0.004, and 0.172 for Mg/CI, Ca/CI, Br/CI and SO[4]/CI, respectively]. The correlation coefficients between the major cations and the hydrographic parameters were computed and discussed

Characterization of triethanolammonium bromide mono-and dibenzoate as cationic surfactants

Novel series of triethanolammonium mono- and dibenzoate bromide cationic surfactants containing different alkyl chains were synthesized. Their surface parameters including, surface tension [gamma], critical micelle concentration [CMC], effectiveness [lambada pi[cmc]], maximum surface excess gamma and minimum surface area [A [min]] were caiculated at 25 degree sign C. Increasing the number of methylene groups in the hydrophobic chains [tails] increases the surface parameters, which decreases the surface tension of these surfactants in their solutions. Energetic parameters of micellization and adsorption of these surfactants in their solutions were also calculated at 25 degree sign C. The results of thermodynamic calculations showed the tendency of these surfactant molecules towards adsorption at the air/water interfaces rather than the micellization in the bulk of their solutions. The surface and energetic parameters were correlated to the ability of these surfactants towards adsorption at the interfaces

Histological study on the toxic effect of 2-bromopropane on the seminiferous tubules of adult male albino rats

2-Bromopropane [2-BP] is a haloalkane used in industry as an alternative to ozone layer depleting solvents. It has recently been suspected to be a causative agent for some reproductive dysfunctions in both male and female workers exposed to it in electronic factories. The study was carried out to evaluate the possible toxic effects of 2-BP administration on the seminiferous tubules [S. Ts] of rat testis, and its potential reversibility after 2-BP withdrawal. The study was conducted on 30 adult male albino rats weighing from 100-150g each. They were categorized into 3 equal groups. Group I: served as a control group. Group II: rats received daily subcutaneous injection of 2-BP [400 mg/kg b.w.] for 28 days. Group III: rats received 2-BP in the same dose and for the same duration as group II followed by a further 28-day recovery period. At the end of experiment, blood samples were collected to detect testosterone levels, and the rat testes were weighed and examined for the daily sperm production. Specimens were taken from the testes of all animals and subjected to both light and electron microscopic examinations. 2-BP administration [in group II] significantly decreased absolute testicular weight and daily sperm production as well as serum testosterone levels. Histologically, atrophy of the S.Ts accompanied by interstitial oedema was evident. Moderate to severe degenerative changes involving all types of spermatogenic cells, including spermatogonia, were also demonstrated. Furthermore, Leydig cells depicted ultrastructural evidence of decreased activity. After a 4-week recovery period [group III], mild increase in serum testosterone levels as well as other laboratory parameters were noticed. However, they were still much less as compared to the control. Histologically, only limited amelioration of the testicular lesions in the germinal epithelium as well as Leydig cells was revealed. Such persistent lesions were attributed to the damaging effect of 2-BP on testicular stem cells; spermatogonia. The study demonstrates vulnerability of the testicular tissue to 2-BP intoxication. Therefore, careful consumption of 2-BP containing solvents and pharmaceuticals is necessary

photo-initiated three step strategy for iridoid monoterpene skeleton

The framework of iridoid monoterpene has been constructed in a photo-initiated three step sequence involving 1,3-cyclo-addition of bromobenzene to vinyl acetate, ring opening of the formed vinylcyclopropyl bromide with Ag[+]/H[2] O[2], and subsequent acid catalysed Hock-cleavage of the generated allylic hydro-peroxide

Tiempos de recuperación del bromuro de rocuronio en diferentes dosis durante la anestesia con sevoflurano en niños / Times of recovery of the recuronium bromide in different doses during the anesthesia with sevoflurane in children

Comparar los tiempos de recuperación del bromuro de rocuronio a 0,3 mg/kg y 0,6 mg/Kg para anestesia general con sevoflurano y óxido nitroso en preescolares y escolares. Previo consentimiento informado, se estudiaron 66 niños, ASA I-II, programados para cirugía electiva, distribuidos en dos grupos, preescolares (2-6 años) y escolares (7-12 años). La inducción anestésica fue inhalatoria con sevoflurano y NO2/O2, intravenosa con fentanyl 1 µg/kg, lidocaína 1,5 mg/kg, propofol 2 mg/kg. La monitorización se hizo por aceleromiografía (TOF-Guard) para la estimulación del nervio cubital. Posterior a la administración el bromuro de rocuronio se registraron los tiempos de recuperación espontánea del D25 y T75; índice de recuperación 25-75 y el TOF al 0,7 y 0,9. Los grupos resultaron estadísticamente homogéneos. Se obtuvieron similares tiempos de recuperación en los preescolares respecto a los escolares, con una prolongación estadísticamente significativa en el T75 a 0,6 mg/kg (P < 0,05) en los preescolares. Se encontró una correlación negativa entre la D25 y la edad, sin diferencia en los otros tiempos medidos

Determination of paracetamol bromopotentio-metrically using silver amalgam and bromide ion-selective electrode

A study of the oxidation of paracetamol with bromine in acetate buffer media has been carried out using potentio bromometric techniques. The different variables such as ratio of the reactants, temperature, pH and time affecting the microdetermination of paracetamol were investigated. The optimum conditions obtained at [Br[2]] / [p-AAP] = 3.0, T = 303K, pH = 4.0 and t = 5 - 10 min, permit the quantitative determination of paracetamol in the concentration range of 0.113 to 13.61 mg using silver amalgam electrode or in the concentration range of 143 to 638 micro g ml[-1] using bromide ion-selective electrode. Application of the proposed techniques for rapid, simple and direct determination of paracetamol in ten different pharmaceutical preparations was demonstrated

Inactivation of Trypanosoma cruzi dihydrolipoamide dehydrogenase by leukocyte myeloperoxidase systems: role of hypochloride and nitrite related radicals

Dihydrolipoamide dehydrogenase (LADH) from Trypanosoma cruzi, the causative agent of Chagas' disease, was inactivated by treatment with myeloperoxidase (MPO)-dependent systems. LADH lipoamide reductase and diaphorase activities decreased as a function of incubation time and composition of the MPO/H2O2/halide system, a transient increase preceding the loss of diaphorase activity. Iodide, bromide, thiocyanide and chloride were effective components of MPO/H2O2 or MPO/NADH systems. Catalase prevented LADH inactivation by the MPO/NADH/halide systems in agreement with H2O2 production by NADH-supplemented LADH. Thiol compounds (L-cysteine, N-acetylcysteine, penicillamine, N-(2-mercaptopropionylglycine) and Captopril prevented LADH inactivation by the MPO/H2O2/NaCl system and by NaOCl, thus supporting HOCl as agent of the MPO/H2O2/NaCl system. MPO/H2O2/NaNO2 and MPO/NADH/NaNO2 inactivated LADH, the reaction being prevented by MPO inhibitors and thiol compounds. T. cruzi LADH was affected by MPO-dependent systems like myocardial LADH, allowance being made for the variation of the diaphorase activity and the greater sensitivity of the T. cruzi enzyme to MPO/H2O2/halide systems.

Estudio comparativo por Espirometría de la modificación de la función ventilatoria con Salbutamol, Bromuro de Ipratropio y su combinación en adultos con diagnóstico de Asma Bronquial, Hospital IPSS-Ilo, 1998

Salbutamol y Bromuro de Ipratropio son dos broncodilatadores con acción a distinto nivel de la vía aérea. Debido a la existencia de reportes conflictivos acerca de la utilidad de adicionar un anticolinérgico al Beta agonista, se realizó el presente estudio para comparar la efectividad de Salbutamol más bromuro de ipratropio en comparación con su uso individual en la mejoría de la función ventilatoria medida por espirometría en pacientes con diagnóstico de Asma Bronquial que acuden a programas externos del Hospital Ipss-Ilo. Se utilizó un diseño de tipo cuasiexperimental. Se estudiaron 30 pacientes (20 mujeres y 10 varones) de 15 a 45 años de edad. Se valoró la función ventilatoria por espirometría y se investigó la modificación de la misma con Salbutamol, bromuro de iparatropio y la combinación de las dos drogas. Para ello se realizó una esperometría basal y luego post-broncodilatadora, con 300 ug. de ASalbutamol, 40 mg. de bromuro de ipratropio y las dos drogas combinadas (a la misma dosis) en forma inhalatoria con una aerocámara. Se encontró que salbutamol, bromuro de ipratropio y salbutamol más bromuro de ipratropio alcanzaron mejoría altamente significativa de los paramétros espirométricos (a excepción del PEF máx. para bromuro de ipratropio, que no fue significativa). La comparación de las drogas mostró que aunque la combinación produjo un porcentaje de cambio superior a salbutamol y bromuro de ipratropio sólos, este no fue estadísticamente significativo. En el presente trabajo se concluye que la asociación de salbutamol más bromuro de ipratropio tiene efecto aditivo en la reversión de la obstrucción bronquial medida por esperometría en pacientes asmáticos aunque esta no es estadísticamente significativa...

Asma inducida por ejercicio, efecto preventivo de la asociación de sulfato de salbutamol y bromuro de ipratropio / Asthma induced by exercise, preventive effect of the association of salbutamol sulfate and ipratropico bromide

Objetivos: El presente es un estudio abierto, prospectivo, que evalúa la eficacia de la combinación fija de sulfato de salbutamol (100 mcg) asociada a bromuro de ipratropio (20 mcg) en inhalador (Combivent) para prevenir el asma inducida por ejercicio (AIE) en niños con edades entre 5 y 14 años. Material y método: Se incluyeron un total de 30 pacientes asmáticos con diagnóstico previo de AIE. Se realizaron tres pruebas de ejercicio previamente estandarizadas en cada paciente, mediante una carrera libre en espacio abierto, considerando la prueba positiva cuando se produjo una caída del 13 por ciento o más en la flujometría respecto al valor de cada paciente. Siete días después de la primera prueba y previa autorización escrita por los padres, se les administró dos puff de la combinación fija de sulfato de salbutamol (100 mcg) asociado a bromuro de ipratropico (20 mcg) en inhalador con aerocámara; 15 minutos luego se les sometió a una prueba de ejercicio así como, luego de tres horas. Resultados: Los niños que ingresaron al estudio, tenían entre 6,1 y 12,3 años de edad, todas las pruebas de ejercicio realizadas luego de la medicación fueron negativas, tanto las realizadas a los 15 minutos como luego de tres horas de la administración de al asociación. No se detectaron efectos adversos y ninguno de los pacientes tuvo que ser excluido por no poder realizar la segunda prueba de ejercicio luego de la administración del medicamento. Conclusiones: La combinación fija de sulfato de salbutamol (100 mcg) asociada a bromuro de ipratropio (20 mcg), administrado por inhalador con aerocámara, es capaz de evitar el AIE tanto a los 15 minutos como luego de 3 horas de ser admnistrada.

Evaluación de la combinación fija de sulfato de sabutamol y bromuro de ipratropio en lactantes con sindrome de obstrucción bronquial: estudio piloto / Evaluation of the fixed combination of sabutamol sulfate and ipratropico bromide in lactantes with syndrome of bronchial obstruction: I study pilot

El presente es un estudio piloto, doble ciego, que busca comparar la eficacia de dos esquemas terapéuticos para el manejo del Síndrome de Obstrucción bronquial (SOB) en niños lactantes menores de tres años. En forma aleatoria, diez pacientes recibieron salbutamol (100 ug/inhalación) y ocho recibieron la combinación fija de sulfato de salbutamol (100 ug/inhalación) mas bromuro de ipratropio (10 ug/inhalación) a los 0, 20, 30, 40, 50, 80, 110 minutos de iniciado el tratamiento, ambos de micro dosificador inhalatorio (MDI) mediante el empleo de un espaciador. Se evaluó el efecto clínico de ambos esquemas utilizando el puntaje Bierman y Pierson modificado por Tal, la frecuencia cardiaca y la saturación de oxígeno a los 0, 30, 60, 90 y 120 minutos de iniciada la terapia. Nuestros resultados mostraron una diferencia a los 60 minutos, donde el grupo que empleó la combinación fija logra mejores valores de puntaje clínico. Por otro lado ambos esquemas significativamente el cuadro del paciente. En conclusión, es posible que la asociación logre mejores resultados en algunos pacientes para el manejo del síndrome de obstrucción bronquial.